Healthcare Professional Support Hub

Technical papers, professional apps, practical resources and specialist support

Welcome to the Healthcare Professional Support Hub

This professional section brings together technical discussion papers, Professional Assistance Papers, selected apps, practical resources, and specialist services developed by Jeff Charlton over more than four decades of international work in building forensics, environmental hygiene, remediation, and exposure investigation.

Many of the current papers are informed by Dual Optics Parallax—a framework that compares building and environmental evidence with symptoms, clinical findings, timing, vulnerability and possible exposure pathways. It draws on practical experience of investigating individual and combined environmental hazards, their interaction and the effectiveness of proposed solutions.

The Hub is designed to encourage professional discussion, challenge current assumptions, improve the interpretation of complex evidence, and strengthen collaboration among healthcare, environmental, scientific, and building professionals, and to reflect on possible alternatives to current protocols, which are increasingly shown to have serious limitations. 

The following index introduces the current subject areas, followed by brief reviews of the available technical papers.

Access is currently free for approved professionals. Some advanced apps, reports, training, mentoring and consultancy services may be available separately.

Technical paper index

Current Paper Subjects

Browse current technical paper sections, selected abstracts and joint research notes.

View Current Paper Subjects

Current Technical Paper Sections & Professional Collaboration

Healthcare professionals, scientists and other specialists are invited to comment, contribute evidence or collaborate on future papers and revisions.

Approved contributions may be published with the contributor's name and agreed role. Each paper will be timestamped and version-controlled to record authorship, publication date, revisions and citation details. No substantive contribution will be used without prior agreement and acknowledgement, and no person will be named without written consent.

Selected Abstract

1. Measurement, Testing and Interpretation

Why technically valid tests may still provide incomplete or misleading results when the sampling method, location, timing, particle fraction, extraction process or biological matrix does not match the question being asked.

Measurement Is Not Exposure: Why detection or non-detection cannot automatically be treated as proof of exposure, absence, causation or safety.

Assay Inhibitors, Amplifiers and Matrix Effects: How PCR inhibition, extraction efficiency, dust composition, cross-reactivity and biological matrices may contribute to false-low, false-high or misleadingly precise findings.

The Human Sample Is Not Neutral: How hydration, diet, metabolism, medication, supplements, timing and sample handling may affect urine, stool, blood and biomarker interpretation.

The Complete Confounder Cascade: How uncertainty may accumulate from building investigation through environmental sampling, laboratory analysis and clinical interpretation.

2. Biological Aerosols and Carrier Particles

Whether indoor exposure may involve more than intact fungal spores, pollen grains or readily identifiable organisms, including respirable fragments, mixed aerosols and particles carrying other biological or chemical material.

Pollen Fragmentation and the Trojan Horse Mechanism: A proposed framework examining respirable pollen fragments and their possible role as carrier particles for allergens, endotoxin, fungal fragments and other inflammatory material.

Carrier-and-Cargo Framework for Indoor Biological Aerosols: An integrated model for considering the particle, its associated biological material and the exposure pathway together.

Mineral Fibres, Silicates and Inorganic Dusts as Carriers, Irritants and Analytical Interferents: A discussion of inorganic particles as irritants, carrier surfaces and possible sources of measurement interference.

Organic Dusts, Skin Scales, Textile Fibres and Mixed Indoor Reservoirs: An examination of indoor reservoirs containing biological debris, textile material, skin scales, microorganisms and associated residues.

Joint Research Papers

Pollen-Endotoxin Paper
Authors: Jeff Charlton, David Lark and Vince Neil

Presented by Jeff Charlton at the Singapore IAQ World Conference June 2026, describing the mismeasurement of pollen in terms of vector agent and historic exposure.

3. Hearing, Ear and Sinonasal Pathways

Whether the ear, Eustachian system, upper airway and sinonasal region may be under-considered in environmental exposure investigation.

  • Glue Ear, Otitis Media with Effusion and Indoor Environmental Exposure
  • Sinonasal Co-Colonisation, Airborne Biological Fragments and Ear-Environment Risk
  • Children, Neuroimmune Vulnerability and Upper-Airway-Ear Pathways
  • The Ear-Sinonasal-Neurovestibular Interface in Water-Damaged Buildings
Further Research Pathways

The agreed joint hearing paper on Otitis Media (Glue Ear) is authored by Jeff Charlton and Dr Dayna Edwin.

4. Olfactory, Trigeminal and Neurological Pathways

Olfactory, trigeminal, perineural, respiratory and inflammatory pathways that may not be fully represented by a simple blood-brain barrier model.

  • Olfactory, Trigeminal and Perineural Routes of Environmental Exposure to the Central Nervous System
  • Fine Particles, Olfactory Transport and Blood-Brain Barrier Questions
  • Upper-Airway Deposition, Neural Interfaces and Systemic Inflammatory Signalling

These papers present research questions and proposed mechanisms. They do not claim that an environmental finding proves neurological disease in an individual.

5. Oral, Dental and Water-Related Pathways

Possible relationships between environmental reservoirs, oral exposure, mouth breathing, water biofilms, dental deterioration and microbial findings.

  • Unusual Dental Decay, Water Biofilms and Indoor Environmental Exposure
  • Oral, Dental and Mouth-Breathing Pathways
  • Root-Canal Infection, Microbial Reservoirs and Environmental Interpretation

6. Remediation, Decontamination and Verification

Why killing organisms, applying chemicals or making a surface appear clean may not amount to successful removal of biologically relevant contamination.

  • When Remediation Is Not Remediation
  • Disinfection Does Not Equal Decontamination
  • Post-Remediation Verification: Why Visual Cleanliness and One Negative Test Are Not Clearance
  • Airborne Mycotoxins and Human Risk
  • Asbestos-Mould Co-Contamination and the Limits of Single-Hazard Clearance

Note on Dual Optics Parallax

A framework comparing building, environmental and exposure evidence with healthcare, symptom, timing and vulnerability evidence. The purpose is to examine alignment, contradiction and uncertainty without treating either evidence stream as absolute proof.

  • Dual Optics Parallax for Building-Related Health Investigation
  • Dual Optics Parallax and the Standards Gap
  • Environmental Data and Clinical Interpretation
  • From Building Evidence to Health-Relevance Assessment

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